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For Enzymatic Hydrolysis of Protein CAS 9002-07-7 Recombinant Trypsin

Categories Recombinant Trypsin
Brand Name: YaxinBio
Model Number: RPT0201
Certification: ISO9001
Place of Origin: Shanghai,China
MOQ: 10mg
Price: 65$/10mg
Payment Terms: T/T
Supply Ability: 1000g per year
Delivery Time: 7 working days after received your payment
Packaging Details: 1.Inside plastic bag and outside moisture-proof aluminum foil packing .2. A special cool incubator to keep the low temperature in transportation,with ice pack
Product Name: Recombinant Trypsin
Size Of Panel: 10mg,100mg,1g or bulk
Source: E. Coli
Purified by: HPLC
Specific activity: ≥3800 USP units/mg pro
Protein content: 35% ~ 70%
Additives: Carbohydrates
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For Enzymatic Hydrolysis of Protein CAS 9002-07-7 Recombinant Trypsin

For Enzymatic Hydrolysis of Protein CAS 9002-07-7 Recombinant Trypsin


Description


Trypsin
Enzyme Commission (EC) Number: 3.4.21.4
Molecular Weight: 23.3 kDa (bovine & porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal

end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The

enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,

e.g.EDTA.Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid

residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage

site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site.

Trypsin will hydrolyze ester and amide linkages of several synthetic substrates.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

Assay Method

The activity of most preparations is determined by a continuous rate spectrophotometric assay

and expressed in BAEE units.

Unit Definition: One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE

as substrate. Reaction volume = 3.2 mL (1 cm light path).


Recommend Usage


Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.


Storage Instructions


Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months

and above 90% activity remained after 10 times repeated freezing and thawing.


Product Tags:

porcine trypsin

  

cell culture trypsin

  
Quality For Enzymatic Hydrolysis of Protein CAS 9002-07-7 Recombinant Trypsin for sale
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