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Stable Recombinant Carboxypeptidase B E.coli Carboxypeptidase B E.coli

Categories Recombinant Carboxypeptidase B
Brand Name: YaxinBio
Model Number: RCPB01
Certification: ISO 9001
Place of Origin: China
MOQ: 10mg
Payment Terms: T/T
Packaging Details: ice packaging
Price: 150$/10mg
Supply Ability: 100000g per year
Delivery Time: 7 work days
Product: Recombinant Carboxypeptidase B
Manufacturer: YaxinBio
Packaging: 10mg,100mg,1g,bulk
Source: E. Coli
Purified by: HPLC
Purity: > 95%
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Stable Recombinant Carboxypeptidase B E.coli Carboxypeptidase B E.coli

Stable Recombinant Carboxypeptidase B E.coli Carboxypeptidase B E.coli


Description
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 33.8 kDa. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Synonyms:carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5
GeneID:113936
mRNA:Refseq
NM_053617
Protein Refseq
NP_446069
UniProt ID
Q9EQV9
Chromosome Location:15q11
Pathway:Compl

Advantages


Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B

Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.


Main Features


Format

White or white-like or yellowish lyophilized

Additives

Tris salts, NaCl salts and carbohydrates

Protein content

35% ~ 60%

Specific activity

≥170 units/mg pro.

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.


Unit Definition


One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.


Recommend Usage


To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

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