Enzyme Trypsin Serine Lysine Protease Arginine Protease Recombinant Trypsin

Enzyme Trypsin Serine Lysine Protease Arginine Protease Recombinant Trypsin

Description

Trypsin can autocatalytically activate more trypsinogen to trypsin. Trypsin consists of a single chain polypeptide of 223 amino acid residues. This native form of trypsin is refered to as β-trypsin. Autolysis

of β-trypsin (which is cleaved at Lys- Ser in the bovine sequence) results in α-trypsin which is held together by disulfide bridges. Trypsin is a member of the serine protease S1 family. The active site

amino acid residues of trypsin include His and Ser

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal

end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The

enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,

e.g.EDTA.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

Advantages

Animal origin free: The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant

Porcine Trypsin belongs to the AOF level 3.

Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

Main Features

Unit Definition

One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

Recommend Usage

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.

Storage Instructions

Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and

above 90% activity remained after 10 times repeated freezing and thawing.

Email:Zhaozhi@yaxinbio.com

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.