Categories | Recombinant Trypsin |
---|---|
Brand Name: | YaxinBio |
Model Number: | RPT0201 |
Certification: | ISO9001 |
Place of Origin: | China |
MOQ: | 10mg |
Price: | 65$/10mg |
Payment Terms: | T/T |
Packaging Details: | ice packaging |
Supply Ability: | 1000g per week |
Product: | Recombinant Porcine Trypsin |
Cat. No.: | RPT0201 |
Specific activity: | ≥3800 USP u/mg pro |
Purity: | > 99% |
Related Product: | Recombinant Lipase A |
Company Info. |
Shanghai Yaxin Biotechnology Co.,Ltd. |
Verified Supplier |
View Contact Details |
Product List |
Enzyme Trypsin Serine Lysine Protease Arginine Protease Recombinant Trypsin
Description
Trypsin can autocatalytically activate more trypsinogen to trypsin. Trypsin consists of a single chain polypeptide of 223 amino acid residues. This native form of trypsin is refered to as β-trypsin. Autolysis
of β-trypsin (which is cleaved at Lys- Ser in the bovine sequence) results in α-trypsin which is held together by disulfide bridges. Trypsin is a member of the serine protease S1 family. The active site
amino acid residues of trypsin include His and Ser
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal
end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The
enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents,
e.g.EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified
by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
Advantages
Animal origin free: The use of recombinant Porcine Trypsin eliminates the risk of virus presence,
and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant
Porcine Trypsin belongs to the AOF level 3.
Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Main Features
Source | E. Coli |
Purified by | HPLC |
Format | White lyophilized |
Purity(RP-HPLC) | NLT 70% β-trysin, NMT 20% α-trypsin |
Contaminant activity | No chymotrypsin, carboxypeptidase A, and other protease contaminant. |
Unit Definition
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Recommend Usage
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Storage Instructions
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable
within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and thawing.
Email:Zhaozhi@yaxinbio.com
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